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tSNE analysis of megakaryocytes (A) Gating strategy post concatenation. Segregation of samples based on treatment function, gate for red blood cells (CD45 vs CD235), megakaryocytes (CD45 vs <t>CD41/CD61)</t> and subsequently gated for maturation marker CD42. (B) Selection of parameters for generating a tSNE plot. (C) Example of the two samples files generated into tSNE plot. (D) Automatic overlay of markers of interest in the panel. (E) Legend for changing colors. (F) Interpretation of results for the samples.
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tSNE analysis of megakaryocytes (A) Gating strategy post concatenation. Segregation of samples based on treatment function, gate for red blood cells (CD45 vs CD235), megakaryocytes (CD45 vs <t>CD41/CD61)</t> and subsequently gated for maturation marker CD42. (B) Selection of parameters for generating a tSNE plot. (C) Example of the two samples files generated into tSNE plot. (D) Automatic overlay of markers of interest in the panel. (E) Legend for changing colors. (F) Interpretation of results for the samples.
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tSNE analysis of megakaryocytes (A) Gating strategy post concatenation. Segregation of samples based on treatment function, gate for red blood cells (CD45 vs CD235), megakaryocytes (CD45 vs <t>CD41/CD61)</t> and subsequently gated for maturation marker CD42. (B) Selection of parameters for generating a tSNE plot. (C) Example of the two samples files generated into tSNE plot. (D) Automatic overlay of markers of interest in the panel. (E) Legend for changing colors. (F) Interpretation of results for the samples.
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Image Search Results


tSNE analysis of megakaryocytes (A) Gating strategy post concatenation. Segregation of samples based on treatment function, gate for red blood cells (CD45 vs CD235), megakaryocytes (CD45 vs CD41/CD61) and subsequently gated for maturation marker CD42. (B) Selection of parameters for generating a tSNE plot. (C) Example of the two samples files generated into tSNE plot. (D) Automatic overlay of markers of interest in the panel. (E) Legend for changing colors. (F) Interpretation of results for the samples.

Journal: STAR Protocols

Article Title: Protocol for generating megakaryocytes from patient induced pluripotent stem cells for disease modeling and compound screening

doi: 10.1016/j.xpro.2026.104393

Figure Lengend Snippet: tSNE analysis of megakaryocytes (A) Gating strategy post concatenation. Segregation of samples based on treatment function, gate for red blood cells (CD45 vs CD235), megakaryocytes (CD45 vs CD41/CD61) and subsequently gated for maturation marker CD42. (B) Selection of parameters for generating a tSNE plot. (C) Example of the two samples files generated into tSNE plot. (D) Automatic overlay of markers of interest in the panel. (E) Legend for changing colors. (F) Interpretation of results for the samples.

Article Snippet: Anti-human CD41/CD61 antibody (FITC); 1:50 , Miltenyi Biotec , CAT#130-124-887, RRID: AB_2819707.

Techniques: Marker, Selection, Generated